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FASTA and FASTQ files may be uploaded via the REST API or by using the One Codex CLI or Python client library. The latter two options are simpler and thus recommended (and also support automatic interleaving and validation of your FASTA/Qs). In every case, we replace spaces in filenames with underscores. To upload with the CLI, simply:
Uploading via the REST API consists of 3 steps:

Samples over 20GB

The REST upload above only allows sample uploads up to 20GB (optionally compressed with gzip). If you need to upload a compressed file over 20GB, please use the onecodex CLI.